| 摘要 |
Process for the preparation of hpGRF via fragments, characterised in that there are coupled, one after the other and in order of sequence, the fragments in which: a. The side acid functional groups of aspartic and glutamic acids and the side amine functional group of lysine are protected by protecting groups which are stable under the conditions for the deprotection of the Boc group; b. The guanidine functional group of arginine is protected by protonation; and c. The N-terminal amino acid is protected on the amine by the Boc group, the Boc group being selectively removed from the N-terminal amine of the peptide in the elongation phase by hydrolysis with trifluoroacetic acid, the said coupling being carried out in an aprotic polar solvent and all the protecting groups are removed at the end of the sequence by hydrolysis using a 0.1 to 1M solution of methanesulphonic or trifluoromethanesulphonic acid in trifluoroacetic acid.
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