Composition, analytical element and method for the quantification of creatine kinase.

摘要

A method is described for the analytical determination of creatine kinase in an aqueous liquid, such as blood serum. This method comprises:
(A) contacting (1) a sample of the liquid to be analyzed, and (2) reagents which effect in the presence of a creatine kinase-containing liquid, an ordered sequence of reactions wherein: (a) creatine phosphate and adenosine diphosphate react in the presence of creatine kinase to form creatine and adenosine triphosphate; (b) glycerol is phosphorylated in the presence of glycerol kinase and adenosine triphosphate to form L- alpha -glycerophosphate; and (c) L- alpha -glycerophosphate is oxidized in the presence of alpha -glycerophosphate oxidase and a colorimetric indicator composition to produce an optical density change; and
(B) quantitatively detecting the rate of such change.
This method can be practiced in solution or on "dry" analytical elements containing a plurality of zones.