DNA expression vectors useful in bacillus cells.

摘要

Recombinant DNA expression vectors for use in Bacillus and other host cells are disclosed. The vectors comprise a Bacillus subtilis ribosome binding site-encoding DNA fragment ligated to the veg promoter of B. subtilis and to a gene that encodes a functional polypeptide, the DNA fragment sequence being <IMAGE> wherein A is deoxyadenyl, G is deoxyguanyl, C is deoxycytosyl, T is thymidyl, R is G or C and R min is G or C, subject to the limitation that R and R min are not simultaneously the same deoxyribonucleotide.