| 摘要 |
PURPOSE:To produce a remarkable amount of L-phenylalanine, by cultivating a microorganism, belonging to the genus Bacillus, having the resistance to N,N- bisphosphonomethylglycine and N-phosphonomethylglycine and ability to produce the L-phenylalanine. CONSTITUTION:A wild strain of the genus Bacillus or the well-known strain capable of producing phenylalanine as a parent strain is subjected to the variation treatment, and scattered on an agar plate culture medium containing N,N- bisphosphonomethylglycine or N-phosphonomethylglycine in such a concentration as not to grown the parent strain to obtain Bacillus subtilis AJ12000, Bacillus subtilis AJ12001 or Bacillus subtilis AJ12002 from the grown colony. The resultant microorganisms are then cultivated in a liquid culture medium to produce a remarkable amount of L-phenylalanine. |
