PEPTIDAS SPECIFIK FOER N-TERMINAL MENTIONIN, PRODUCERING OCH ANVAENDNING AV DENSAMMA.

摘要

Methods of obtaining N-terminal methionine-free proteins are described. The methods employ a novel enzyme, methionine aminopeptidase (e.g. from E. coli) either in vitro or in vivo. For in vivo application, plasmid-borne DNA encoding the peptidase is transformed into a bacterial host which produces the desired protein.