| 摘要 |
PURPOSE:To obtain a column which stably holds a solid phase and has excellent separation capacity, by forming an organopolysiloxane type crosslinked high- molecular membrane to the inner surface of the column for chromatography while applying a fixing phase liquid to the surface of said membrane. CONSTITUTION:A volatile solvent solution prepared by adding a radical generating agent such as benzoyl proxide to organopolysiloxane having one or more of vinyl groups such as methylphenylvinylsilicone to the inner surface of the tube 1 for chromatography comprising soda lime glass, borosilicate glass or stainless steel and dried and heated for a time sufficient to form a crosslinked membrane 2. In the next step, a solution of non-polar or polar methylphenylsilicone is applied to said membrane 2 and a solvent is removed to form a uniform fixing phase liquid coating 3. As mentioned above, surface roughening treatment such as chemical etching of an internal surface becomes unnecessary and excellent separation capacity in which the feature of the inert surface possessed by quartz glass is usefully adapted can be provided, especially, to the capillary tube made of quartz glass. |
