Method for fusion of plant protoplast.

摘要

A method for fusing plant protoplasts comprising the steps of; (a) treating the plant protoplasts with a fusing agent, and then (b) incubating the resultant plant protoplasts in an incubation medium having an osmotic pressure lower that that required for maintaining the plant protoplasts in a stable form. This effectively promotes the fusion of the plant protoplasts at a higher fusion rate in a wider range of plants.