Process for the recovery of human beta -interferon-like polypeptides

摘要

An improved process for recovering and purifying beta -HIFN from transformed bacterial comprising concentrating the bacteria; disrupting the cell wall and solubilizing the beta -HIFN into an aqueous medium with an appropriate solubilizing agent; extracting the beta -HIFN from the aqueous medium with 2-butanol, 2-methyl-2-butanol or mixtures thereof; precipitating and isolating the beta -HIFN from the alcohol phase; purifying the beta -HIFN by chromatography and diafiltering the beta -HIFN against distilled water or aqueous solutions of ethanol or glycerol at a pH of about 12; and therapeutic formulations and compositions of beta -HIFN within SDS levels therein reduced to less than 10 p.p.m.