| 摘要 |
The invention provides enzyme immunoassays whose sensitivity is increased by cascade amplification. The coupled ligand (enzyme or an activator) catalytically activates a second enzyme which acts on a substrate or can act on a third enzyme to produce a cascade. Alternatively, a proenzyme is coupled to the ligand and converted by an activator to an enzyme which is itself an activator of a second proenzyme in a cascade.
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