Signal processing method in autoradiography.

摘要

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing groups of radioactively labeled base specific cleavage products or mixture thereof obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element and resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium, which comprises a process including:
(1) detecting sampling points in each resolved row;(2) determining reference sampling points in each reference row which is practically provided on the support medium and/or synthesized from the resolved rows;(3) joining the corresponding reference sampling points among said plural reference rows to fix plural continuous lines comprising straight lines, polygonal lines or curved lines; and(4) comparing and identifying said sampling points in each resolved row utilizing said continuous lines;
said process being applied to a digital signal corresponding to an autoradiograph having the locational information on the groups of radioactively labeled cleavage products, said digital signal being obtained from the autoradiograph visualized on a radiosensitive material.