发明名称 |
PROCESS FOR THE ENZYME IMMUNO DETERMINATION IN THE HETEROGENOUS PHASE |
摘要 |
<p>The present invention provides a process for the determination of a component of the reaction between a specific binding protein and a substance bindable therewith by incubation of a sample solution with a previously ascertained amount of binding component in insoluble form in the presence of a reagent which contains a known amount of one of the binding components in enzyme-labeled form, separation of the liquid phase from the solid phase by centrifuging and determination of the activity of the marker enzyme remaining in the liquid phase, wherein measurement of the enzyme activity is carried out in a centrifuge rotor during or after the action of increased gravitational force whereby the solid phase and the liquid phase are still in contact with one another.</p> |
申请公布号 |
CA1170180(A) |
申请公布日期 |
1984.07.03 |
申请号 |
CA19810391389 |
申请日期 |
1981.12.02 |
申请人 |
BOEHRINGER MANNHEIM G.M.B.H. |
发明人 |
EVANEGA, GEORGE R.;ALBERT, WINFRIED |
分类号 |
G01N33/543;(IPC1-7):G01N33/54;C12Q1/00 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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