| 摘要 |
The enzyme fixed to a support is composed of a combined organic-inorganic base material, which consists of an inorganic porous support having an organic polymer, which contains functional groups, partially adsorbed and enclosed in its pores, and of an enzyme which is partially adsorbed to the base material and bound covalently to the functional groups of the organic polymer in the terminal regions of this polymer. Such immobilised enzymes are of use in the biochemical and fermentation industries. They are obtained, in particular, by drenching a porous support with a solution of a polyfunctional organic compound and treating the impregnated support with an excess of a bifunctional monomer. Subsequently, the enzyme is added, part of which enzyme then binds covalently to reactive groups of the polymer which has been formed and which is present in and on the support. During this procedure, the biochemically active residues of the enzyme must not be blocked or altered. |
