| 摘要 |
A purified vascular permeability protein factor and method of manufacture, wherein the factor has the following characteristics: (a) in an aqueous solution (0.01 M Na3PO4, pH 7) whose concentration of NaCl is varied linearly, the factor is eluted from a heparin-Sepharose chromatography column in a peak centered at 0.4 M NaCl; (b) in an aqueous solution of Na3PO4 (pH 7.0) whose concentration is varied linearly, the factor is eluted from a hydroxylapatite column in a peak centered at 0.25 M Na3PO4; (c) when subjected to SDS gel electrophoresis in a 7.5% polyacrylamide slab gel (0.375 M tris-HCl (pH 8.8), 0.1% SDS) at 35 milliamps and 4 DEG C., the factor is localized to a region corresponding to a molecular weight between 34,000 and 45,000 daltons. |
