Process for purifying and separating vatimin B12.

摘要

A process for the separation of vitamin B12 in pure form which comprises bringing a vitamin B12-containing liquid having impurities into contact with a divinylbenzene styrene copolymer resin, and causing vitamin B12 to be adsorbed to the resin. The vitamin B12 thus adsorbed is eluted an eluent which is an aqueous solution containing a compound selected from the group consisting of a lower ketone, a lower ester and a lower ether. The resin may be treated before elution with purified water or an aqueous low-acidity solution.