| 摘要 |
A concept and various means of immunological assay are disclosed wherein two different classes of particles which interact at short distances to produce characteristic detectable signals are employed in a modification of the usual latex fixation test. In one embodiment of the concept an aqueous suspension of appropriately coated tritiated latex particles (LH) and polystyrene scintillant particles (L*) is employed. The amount of (LH) (L*) dimer formation and higher order aggregation induced and therefore the concentration of antibody (or antigen) present which caused the aggregation can be determined by using standard liquid scintillation counting equipment. Also, an optical container for use in the assay which has one side provided with a section forming a quadrilateral prism with the prism providing for an angle of incidence greater than the critical angle for an electromagnetic beam of radiation.
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