| 摘要 |
PURPOSE:To collect glucan having improved carcinostatic activity in high yield, by subjecting a culture fluid of Cordyceps ophioglossoides Fr, to a specific purification treatment. CONSTITUTION:(i) Cordyceps ophioglossoides Fr. (available from the Institute for Fermentation, Osaka) is cultivated in an agar plate culture medium to grow mycelia on the surface thereof, and the resultant mycelia are cultivated in a liquid culture medium at about 20-30 deg.C. (ii) The resultant highly viscous culture is mixed with distilled water in an amount of about 2-3 times of that of the culture and then centrifuged to remove the mycelia. The resultant cultivation filtrate is then dialyzed to remove low-molecular weight substances (having <=1,000 molecular weight). (iii) An equal amount of ethanol is added to the resultant cultivation filtrate, and the resultant mixture is allowed to stand in a cold place overnight to precipitate crude glucan, which is dissolved in an aqueous solution of acetic acid. The resultant solution is then kept at 35-100 deg.C to deposit the glucan. After cooling the solution, the glucan is collected, washed with water and dried under reduced pressure to afford the aimed purified glucan powder. |
