发明名称 |
FREMGANGSMAADE TIL FREMSTILLING AF EN REKOMBINANT BACULOVIRUS-UDTRYKKELSESVEKTOR |
摘要 |
A method for producing a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell, is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant transfer vector is prepared by inserting said DNA fragment into a cloning vehicle and there-after inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant transfer fector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. The resultant recombinant baculovirus is then used to infect susceptible insects or cultured insect cells and the protein product from the incorporated selected gene is produced from the infection. |
申请公布号 |
DK257984(D0) |
申请公布日期 |
1984.05.25 |
申请号 |
DK19840002579 |
申请日期 |
1984.05.25 |
申请人 |
THE *TEXAS A&M UNIVERSITY SYSTEM |
发明人 |
GALE E. *SMITH;MAX D. *SUMMERS |
分类号 |
C12N15/09;C07H21/04;C07K14/01;C07K14/52;C12N5/10;C12N15/00;C12N15/62;C12N15/86;C12N15/866;C12P21/00;C12P21/02;C12R1/91;C12R1/92 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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