| 摘要 |
<p>DNA fragments from Treponema pallidum are packaged into suitable bacteriophage vectors for propagation within E. Coli. The resulting plaques are screened for antigens reactive with antibodies to Treponema pallidum. Clones from positive plaques are further propagated and purified to provide antigens useful in the serodiagnosis and study of syphilis and as vaccines against syphilis infection. Plasmid subclones of treponemal antigen producing phage clones are propagated in E. coli to provide an alternate source of treponemal antigens. In addition to bacteriophage clones which encode for polypeptide treponemal antigens, a clone is also disclosed which encodes for a protease resistent heat labile treponemal antigen. </p> |
