| 摘要 |
Rosamicin (I; antibiotic 67-694) was prepd. by fermn. with Micromonospora rosaria RK-2. Thus, a wild type of M.rosaria was treated with MNNG (N-methyl-N'-nitro-N-nitrosoguanidine) to give its RK-2 mutant, which was cultured in a medium contg. saccharose 4, L-asparagin 0.15, KNO3 0.3, CaCO3 0.1, K2HPO4 0.2, MgSO4.7H2O 0.05, FeSO4.7H2O 0.0028, CUSO4.5H2O 0.00025, MnSO4.(4-6)H2O 0.00026, ZnSO4 0.0003, and NaCl 0.01% at 28≦̸C and 280 rpm for 5 days. The accumulation of I reached 300 g/mL after 5 days.
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