REARRANGED DNA MOLECULE FOR PRODUCING B-TYPE HEPATITIS CELL SURFACE ANTIGEN

摘要

Described are a) recombinatn DNA molecules containing a DNA fragment from Hepatitis B Virus comprising the structural gene of the Hepatitis B surface antigen; b) recombinant cloning vehicles containing said recombinant DNA molecules; c) microbial hosts containing said recombinant cloning vehicles; d) genomes or parts thereof of animal viruses as eukaryotic vectors containing said recombinant DNA molecules or recombinant cloning vehicles for selection of vertebrate cells and expression of at least one Hepatits B surface antigen in the selected cells; e) established vertebrate cell lines continuously producing in a nutrient medium large amounts of at least one Hepatitis B surface antigen which is released into the nutrient medium; f) compositions containing at least one Hepatitis B surface antigen as produced by said cell lines for stimulating the production of antibodies in humans to Hepatitis B Virus infection or for detecting Hepatitis B Virus infection or antibodies to Hepatitis B Virus.