| 摘要 |
PURPOSE:To separate and refine a large amt. of sample in a short time with good accuracy by holding one of two separated liquid phases as a stationary phase by centrifugal force, passing the other as a mobile phase continuously through the stationary phase and collecting the sample in the mobile phase by fractionation. CONSTITUTION:One of proportionated liquids separated to two phases is filled as a stationary phase liquid in a column cartridge 7 and a centrifugal acceleration is applied by rotating a rotor 8. The other is fed as a mobile phase liquid from a tank 1 through a joint 5 to the cartridge 7; at the same time, a sample soln. is fed into the cartridge from an injector 3. The sepn. of respective components is accomplished between the mobile phase liquid and the stationary phase liquid. The separated components are flowed out together with the mobile phase liquid from the joint 5. The sample components in the mobile phase liquid flowed out therefrom are detected with a UV absorption monitor 14 and are recorded in a recorder 15, and are dispensed by each specified amt. with a fraction collector 16. The mobile phase liquid flowed out from the monitor 14 is returned to the tank 1. The liquid in the tank 1 is flowed back as the stationary phase liquid and the components in the stationary phase liquid are detected. |
