| 摘要 |
PURPOSE:To eliminate a purifying process of enzyme, to recover an immobilized mold and to reuse it, by bringing the immobilized mold of a bacterium containing an enzyme capable of decomposing chondroitin sulfuric acid into contact with chondroitin sulfuric acid and separating a formed chondroitin sulfuric acid decomposition product. CONSTITUTION:A mold obtained by growing a bacterium such as Proteus vulgaris, etc., containing an enzyme capable of decomposing chondroitin sulfuric acid in a nutritive medium or a mold obtained by further treating the mold in a derived medium is immobilized, hardened and molds between lattices are destroyed by a physical and chemical means. The immobilized mold is brought into contact with chondroitin sulfuric acid and a chondroitin sulfuric acid decomposition product is obtained. A solution having finished the reaction or a column effluent is passed through a molecular sieve and unreacted chondroitin sulfuric acid, etc., are readily separated. |
