| 摘要 |
PURPOSE:To enable an accurate measurement of the density of an antigen by arranging the action of an insoluble antibody in the presence of serum protein and polyalkylene glycol when the insoluble antibody specifically to be bonded to a measuring material (anitgen or the like) and an antibody labelled by an enzyme are allowed to react upon the measuring substance. CONSTITUTION:An antibody (b) supported on an insoluble carrier and an isolated antibody (d) labelled by an enzyme is made to react upon a measuring substance (a) (antigen, hapten or the like) to measure the activity of the enzyme in a bond type labelled antibody d1 by separating the antibody d1 in the insoluble antibody-the measuring substance-antibody from an isolated antibody d2 through competition reaction between the antibodies (b) and (d), serum protein obtained from a bovine, horse, goat or the like and a compound with an average molecular weight of 1,000-2,000 such as polyethylene glycol and polypropylene glycol are added to the reaction system. This permits the absorbance of coloring and fluorescence to be presented as shown by the curve A in stead of in the inverted-V pattern as shown by the curve B as generated in the reaction between the enzyme and the substrate when the density of the measuring substance is high thereby enabling an accurate measurement of the density. |
