摘要 |
PURPOSE:To separate minutely a sample liquid into isoelectric point graduation, by arranging a liquid sending side slender tube row for electrophoresis separation along an upper verge of an electrophoresis separation vessel and also, arranging a sample dispensing side slender tube along a lower verge of it, and dispensing by sending the sample liquid and migrating at a sample's own isoelectric point. CONSTITUTION:Whole tube rows 7, 8 consisting of plural slender tubes are arranged facing each other along both upper and lower facing verges of an electrophoresis separation vessel and a liquid for migrating separation is circulated with a pump Po by using the slender tube row 7 for a liquid sending side for migrating separation and also, using the slender tube row 8 for a dispensing side and then, the sample dispensing liquid is dispensed together with the liquid for separation into test tubes 61, 62... by a valve 10 of the dispensing side slender tube row. A synthetic amphoteric electrolyte (an ampholite solution) is used for the liquid for migrating separation and circulated, while conducting electricity by electrodes 5, 5'. After a gradient of hydrogen ion concentration is imparted sufficiently, a sample liquid 4 is sent by a pump P and is dispensed at any time by circulating it. |