发明名称 METHOD AND APPARATUS FOR IMPARTING HYDROGEN ION CONCENTRATION GRADIENT, MIGRATING AND DISPENSING HYDROGEN ION IN NONCARRIER THIN LAMINAR FLOW ISOELECTRIC POINT ELECTROPHRESIS
摘要 PURPOSE:To separate minutely a sample liquid into isoelectric point graduation, by arranging a liquid sending side slender tube row for electrophoresis separation along an upper verge of an electrophoresis separation vessel and also, arranging a sample dispensing side slender tube along a lower verge of it, and dispensing by sending the sample liquid and migrating at a sample's own isoelectric point. CONSTITUTION:Whole tube rows 7, 8 consisting of plural slender tubes are arranged facing each other along both upper and lower facing verges of an electrophoresis separation vessel and a liquid for migrating separation is circulated with a pump Po by using the slender tube row 7 for a liquid sending side for migrating separation and also, using the slender tube row 8 for a dispensing side and then, the sample dispensing liquid is dispensed together with the liquid for separation into test tubes 61, 62... by a valve 10 of the dispensing side slender tube row. A synthetic amphoteric electrolyte (an ampholite solution) is used for the liquid for migrating separation and circulated, while conducting electricity by electrodes 5, 5'. After a gradient of hydrogen ion concentration is imparted sufficiently, a sample liquid 4 is sent by a pump P and is dispensed at any time by circulating it.
申请公布号 JPS5952743(A) 申请公布日期 1984.03.27
申请号 JP19820163347 申请日期 1982.09.20
申请人 ATOO KK 发明人 YAMADA SHIGEMITSU;IIZUKA TAKASHI
分类号 G01N27/26;G01N27/447 主分类号 G01N27/26
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