| 摘要 |
<p>A method for assaying the direct bilirubin content of a sample comprising (a) mixing a sulfanilic acid solution comprising from about 0.02 to about 0.05 M sulfanilic acid and from about 0.02 to about 0.15 M hydrochloric acid with a sodium nitrite to thereby commence a diazotation process, the ratio of the sulfanilic acid solution to said sodium nitrate solution being preselected so as to yield a diazo solution comprising from about 0.0001 to about 0.001 M Diazo dye; (b) allowing the diazotation process to form the diazo solution for at least 15 seconds; (c) introducing an aliquot of the sample to be assayed into said diazo solution at a volume ratio of from about 1:100 to about 1:25 to thereby commence an azobilirubin process; and (d) measuring the instantaneous rate of azobilirubin formation at 545 nm in a time range of from about 12 to about 25 seconds after introduction of the aliquot of sample into the diazo solution. </p> |
