| 摘要 |
A method is provided for converting extended volumes of mixtures of proteins, or other ampholytes, into an isoelectric spectrum, or sorting pattern, of sharp narrow ampholyte zones located at points of a pH gradient corresponding to the isoelectric pH value of the individual ampholytes. A temperature gradient is established in an electrophoretic buffer column, and the temperature gradient provides a stable pH gradient which can be easily controlled by variations of the temperature limits, and which remains stable when an electric current is passed through the buffer column.
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