摘要 |
PURPOSE:To prepare L-isoleucine compound in high efficiency, by the microbial action using dl-alpha-keto-beta-methylvaleric acid as a raw material. CONSTITUTION:A microbial strain belonging to Bacillus genus or Micrococcus genus, etc. and capable of converting dl-alpha-keto-beta-methylvaleric acid to an L- isoleucine compound, e.g. Bacillus subtilis IFO-3073, is cultured, and the cultured product, cultured cell, or treated cell is made to react with dl-alpha-keto-beta- methylvaleric acid or its salt in an aqueous buffer solution containing ammonium salt of inorganic acid or organic acid or urea, at 5.0-11pH and 15-60 deg.C, and the produced L-isoleucine compound is separated from the product. |