发明名称 |
Methods, compositions and elements for the determination of lipase. |
摘要 |
<p>The present disclosure relates to methods, compositions and elements for the determination of the amount of lipase in a sample. The method comprises the steps of: (a) contacting the sample with a reagent composition comprising: i) a lipase substrate which is a glycerol triester oil having in one of its two alpha -ester positions a long chain alkyl group having at least 8 carbon atoms and, in its two remaining ester positions, short chain alkyl groups such that, if the long chain alkyl group is hydrolyzed, the resulting diester is water soluble; and ii) an esterase enzyme capable of catalyzing the hydrolysis of a water soluble glycerol diester to glycerol; and b) detecting the rate at which glycerol is formed. The compositions of the present invention include the substrate and enzyme as defined. The element of the invention cornprises a support having thereon the described composition. The invention is useful for determining lipase in samples which contain endogenous glycerol, such as blood serum and other body fluids.</p> |
申请公布号 |
EP0101046(A1) |
申请公布日期 |
1984.02.22 |
申请号 |
EP19830107827 |
申请日期 |
1983.08.09 |
申请人 |
EASTMAN KODAK COMPANY |
发明人 |
LIMUTI, CHARLES MICHAEL;MAUCK, JOHN CHARLES;BABB, BRUCE EDWARD |
分类号 |
G01N33/92;C12N9/20;C12Q1/44;C12Q1/61;(IPC1-7):12Q1/44;12N9/20 |
主分类号 |
G01N33/92 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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