SYNTHETIC SUBSTRATE CYTIDINE 3'-PHOSPHATE DERIVATIVE AND DETERMINATION OF RIBONUCLEASE ACTIVITY USING THE SAME

摘要

NEW MATERIAL:The cytidine 3'-phosphate derivative of formula (R is alpha-naphthyl, 4-methylumbelliferyl, p-nitrophenyl, 5-iodo-3-indolyl, or 3-indolyl) and its salt. EXAMPLE:Triethylammonium alpha-naphtyl 3'-cytidylate. USE:A synthetic substrate for the determination of serum ribonuclease activity useful for the diagnosis of pancreatic cancer. It can be determined by an automatic analyzer in a short time. PROCESS:The compound of formula can be prepared by the condensation reaction of N<4>, 2', 5'-protected-3'-cytidylic acid with a color-developing aromatic compound of formula ROH [when R is (5-iodo)-3-indolyl, N-protected compound is used as the raw material](e.g. alpha-naphthol, 4-methylumbelliferone, etc.), followed by the elimination of the protecting group.