| 摘要 |
The present invention relates to a new type of chromatography technique, referred to as dynamic column chromatography, for separation of one or more compounds present in a solution, which is characterized by the existence of a moving solid adsorbent bed. According to the invention, the chromatographic system comprises a piston having at its bottom a sealing element and a longitudinal channel containing the adsorbent between two barriers and a test tube having at its bottom a multiple way valve. By pushing the piston into the test tube, the desired eluent which was prior forced through said valve is entering under intrinsic pressure of the closed system through the channel moving the adsorbed compounds to be separated between said barriers, the solution obtained going out through a nozzle located at one of the end parts of the piston. The dynamic column liquid chromatography is applicable to: silica gel chromatography, reversed phase liquid chromatography, affinity chromatography, capillary chromatography, chromato-focusing, gel filtration and ion exchange chromatography. In the dynamic column liquid chromatography, the equilibrium distribution of the compounds between the adsorbent and liquid is established very rapidly, resulting in sharp and narrow zones of the separated fractions. |
