| 摘要 |
GENETIC ENGINEERING It has been a problem to find an alternative, less timeconsuming, and more reliable source of factor IX, a polypeptide which is essential to the human blood clotting process and necessary for the treatment of patients with Christmas disease. The invention is an important step towards solving the problem by way of genetic engineering, in that it provides recombinant DNA containing a DNA sequence occurring in the human factor IX genome. It includes recombinant DNA comprising substantially the whole sequence of human factor IX genome inserted in a cloning vehicle and transformed into a host such as E.coli. It is conveniently characterised by a 129 or 203- nucleotide long sequence (J-J' and J'-J" in Figure 9). Other fragments of the sequence have also been cloned and the invention includes DNA molecules comprising part or all of the human factor IX DNA. The invention also includes cDNA derived from human factor IX RNA. Uses of the invention include the provision of an intermediate of value in the genetic engineering of a factor IX polypeptide precursor and thence manufacture of the factor IX polypeptide, and in making probes for use in diagnosing the presence of normal or abnormal factor IX DNA in patients with Christmas disease. |
