Enzymatic reaction process.

摘要

A biochemical enzymatic reaction involving an oxidizing enzyme which converts a substrate to an oxidized product with NAD<+> or NADP<+> as coenzyme is provided with a recycling system for returning the NADH or NADPH produced to the oxidized state by the presence of a NADH or NADPH oxidase. The use of the oxidase to recycle the coenzyme avoids the presence of undesirable additional substrates and by-products and in general the oxidation potential of the reverse reaction enhances the basic enzymatic oxidation.