| 摘要 |
<p>The process allows both the hemolysis of the blood cells and the precipitation of the hemoglobin to be carried out in one step while retaining the total Cu, Zn-superoxide dismutase (SoD) activity and the activity of other enzymes, in particular catalase and carbonic acid anhydrase. This is achieved by admixing wholly or partly isolated blood cells with an alkanol having 2 to 4 carbon atoms, preferably ethanol, until a concentration of 10 to 70% by volume and allowing them to stand for hemolysis of the blood cells and denaturation of the hemoglobin, and then adding water at least up to approximately the double volume, removing the precipitate of cell residuals, hemoglobin and other denatured proteins from the suspension, and finally isolating the desired enzymes from the solution obtained.</p> |
