摘要 |
Glutamic acid fermentation process and measurement of cell concentration were automatized by exit gas analysis. Thus, Corynebacterium sp. was inoculated into 13L medium contg. molasses 2.6L, K3PO4 13.0g, MgSO4 5.2g, MnSo4 0.13g, Fe2So4 0.13g, Corn steep liquor 19.5g, and thiamine 2600μg and incubated at 31≦̸C in a 30L fermentor. Parts of the exitgas, according to the cell growth, were passed through CO2-analyzer and O2-analyzer. By the measurement of the produced O2 and CO2, penicillins or surfactants were automatically supplied into the fermentor.
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