<p>A water insoluble, enzymatically active immobilized lactase is provided herein having a high lactose-hydrolyzing activity and possessing industrial advantages. This lactase is produced from Aspergillus oryzae on a carrier of a macroporous phenol-formaldehyde anion-exchange resin. The immobilized lactase enables a continuous catalytic reaction of the hydrolysis of lactose in milk, whey and whey permeate.</p>