| 摘要 |
PURPOSE:To achieve separation and analysis of physiological active substance or medicine with a low molecular weight in a solution containing protein quickly at a high accuracy without removal of protein empolying a reverse phase filler for a liquid chromatograph treated with protein. CONSTITUTION:When physiological active substance, medicine and the like with a low molecular weight existing in a solution containing protein are separated to assay, a reverse phase filler for liquid chromatography pretreated by protein is used for the separation and analysis. The reverse phase filler for liquid chromatography uses a silica gel having a covalent bond of octadigital group, octyl group or phenyl group or a fine particle of a gel having an organic vinyl high molecular frame. The use of a filler thus treated by protein enables a direct and highly accurate measurement without removal of protein for a specimen such as biological liquid. |
