Temperature sensitive multicopy plasmids and organisms containing the same, their production and use in protein production.

摘要

The invention provides plasmids of Co1E1 type parentage which have a temperature sensitive replication system such that the plasmid copy number in a given host cell may be controlled by a temperature shift. Such plasmids permit the use of temperature as a switch for controlling gene amplification and hence the production of corresponding protein in a host cell.