PULSED VOLTAMMETRIC DETECTION OF BACTERIA

摘要

Reliable and rapid detection of microorganism is accomplished in an electroanalytical cell using a pulsed voltammetric detection technique employing the growth medium as the electrolyte and analyte and using simple wire electrodes fabricated from readily available materials. Organism detection occurs as a consequence of the depletion of oxygen in the growth medium/electrolyte caused by aerobic metabolism. Times-to-detection vary with inoculum strength in a predictable fashion, permitting quantification of the organism in question when results are compared to those obtained using known inocula of the same organism. The low duty cycle of the pulsed measurement enables the determination of the relative redox potential in the same cell using the same set of electrodes in order to provide information which may be characteristic of the type of organism being studied.