Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins

摘要

An immunochemical method and reagent compounds for determining ligands in a sample. The reagent compounds are derivatives of a triazinylaminofluorescein and are represented by the structural formula wherein Y is halo or lower alkyl; and R is a ligand-analog wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody. The reagent compound and an antibody specific to the ligand are added to the sample in a fluorescence polarization immunoassay.