| 摘要 |
PURPOSE: To obtain the new gene capable of efficiently producing an active human ALT by modifying a gene in the N-terminal of human ALT (alanineaminotransferase) so as not to cause the substitution of an amino acid, and adding restriction enzyme sites. CONSTITUTION: The method for producing the new active human ALT gene comprises modifying a base sequence coding the N-terminal region of the amino acid sequence of human ALT(alanineaminotransferase) so as not to cause the substitution of an amino acid, and simultaneously adding restriction enzyme sites to the upstream and downstream of the gene. The modified human ALT gene is ligated to a vector, and the prepared plasmid is introduced into Escherichia coli, etc. The transformant is subsequently cultured. Thus, the active human ALT useful as a standard for diagnosing the serum of a hepatic disease, etc., can efficiently be produced. The gene is obtained by cloning by a PCR using a plasmid having a human ALT gene as a template in the presence of a sense primer of formula I and an anti sense primer of formula II. |
