| 摘要 |
PURPOSE:To prepare high-purity interferon useful as a drug economically from an elute which is obtained by adsorbing a crude human interferon solution on an insoluble blue carrier, recovering the elute, adsorbing again the recovered solution on a specific carrier having a chelate group bond. CONSTITUTION:A crude human interferon solution is brought into contact with an insoluble blue carrier (e.g., crosslinked agarose, a carrier, linked to a dyestuff shown by the formula I through ether bond, etc.) at preferably 5-9 pH, so that the interferon is adsorbed on the blue carrier. The interferon is recovered as a solution using the elute, and the recovered solution is brought into contact with a carrier [e.g., insoluble polysaccharide-based carrier shown by the formula II(Me<2+> is Co<2+>, Ni<2+>, or Zn<2+>) obtained by chelating at least one metal selected from Co, Ni, and Zn.] having a chelate group bond at preferably 7-8 pH. Interferon is adsorbed on the carrier, the elute is recovered, and interferon is concentrated and purified.
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