| 摘要 |
<p>DNA sequences comprising structural genes coding for (1) a polypeptide having the amino acid sequence and properties of human calcitonin and for (2) polypeptide analogs thereof which differ in terms of the identity and/or location of one or more amino acids, e.g., ¨ADVal8¨BD and ¨ADAsn15¨BD analogs of human calcitonin. Structural gene sequences may be provided with initial and terminal sequences which facilitate production of discrete protein products by selected host microorganisms as well as for expression by host organisms of fusion proteins, e.g., β-lactamase-calcitonin and β-galactosidase-calcitonin from which the desired products may be isolated. DNA sequences may also be provided with codons specifying additional initial and/or terminal amino acid sequences in polypeptides expressed, which additional amino acids facilitate isolation of active proteins.</p> |
