| 摘要 |
<p>PURPOSE:To carry out preferentially sugar ioversion reaction and to enable the preparation of oligosaccharide in high yield, by feeding a lactose solution having a specific concentration at a specified space velocity to a column packed with a carrier to which beta-galactosidase is immobillized, treating it with the enzyme. CONSTITUTION:Lactose is treated with beta-galactosidase produced by Aspergillus oryzae, to prepare a substance for promoting the propagation of Bifidobacterium, consisting of oligosaccharide as an active ingredient. In this method, beta- galactosidase immobilized on a carrier is used, and the lactose concentration of the lactose solution to be treated is 10-36wt%, and the pH is preferably 3.0- 7.0. The lactose solution is fed at 10-70/hr space velocity to a column packed with the carrier to which beta-galactosidase is immobilized to carry out enzymic treatment. Namely, in the competitive hydrolysis reaction and sugar ionversion reaction in the treatment of lactose with beta-galactosidase, the latter is done preferentially, so that oligosaccharide is obtained in high yield.</p> |
