| 摘要 |
<p>Glutathione is prepared by culturing a microorganism derivable by mutation from a bacterium of the genus Escherichia, said microorganism acting upon a gamma -glutamyl-L-cysteine synthesizing enzyme system and capable of reducing inhibition of gamma -glutamyl-L-cysteine synthetase by glutathione, said culturing being effected to accumulate glutathione in the culture medium and recovering the accumulated glutathione therefrom. The microorganism may also be cultured to accumulate a gamma -glutamyl-L-cysteine synthesizing enzyme system, followed if desired by separation of the enzyme system from the culture medium, reaction of L-glutamic acid, L-cysteine and glycine in the presence of the enzyme system to produce glutathione and separation of the glutathione obtained from the reaction mixture. <??>The microorganisms for use in the processes of the invention are novel and processes for preparing the microorganisms by mutation are described. </p> |
