| 摘要 |
PURPOSE:The titled monoclonal antibody that is obtained by isolating from autoptic livers with non-A and non-B type hepatitis and purifying the product, thus being used as an ingredient of a diagnostic agent for non-A and non-B hapatitis, because of its showing characteristic antigen-antibody reaction with antigens relating to non-A and non-B type hepatitis. CONSTITUTION:The objective monoclonal antibody is obtained by isolating from autoptic livers with non-A and non-B type hepatitis and purifying the product, shows a characteristic reaction with antigens relating to the above hepatitis and has following physical and chemical properties: molecular weight, more than 1,500,000 (by the gel filtration method); sedimentation constant (10<-13>), 51.5S (by the ultracentrifugation method); floating density (g/cm<3>), 1.15-1.25 (in CsCl or KBr); particle size (nm), 26-37; electric mobility, in the alpha2-alpha1 globulin region (in the agarose gel). It is used as a diagnostic agent containing the antibody as a major ingredient, e.g., in the reverse passive hemaggultination method using sheep sensitized erythrocytes or the antibody sandwitch method using sensitized glass beads.
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