| 摘要 |
PURPOSE:To isolate stevioside and rebaudioside A in high purity, by treating crystals of sweetening components of stevia refined in mixture form, with a 3- 6C alcohol. CONSTITUTION:Dried stalks and leaves of stevia are extracted with cold water, hot water, hydrous alcohol, etc., the extract is coagulated or precipitated with an adsorbent, and the precipitate, etc. are removed by filtration, centrifugal separation, etc. to obtain a clear liquid containing the sweetening components. The components are adsorbed to a synthetic polymer adsorbent, purified to 80-90% purity, concentrated, dried, and dissolved in 3-8 times volume of hot methanol or hot ethanol. Stevioside and rebaudioside A are crystallized from the solution simultaneously. After removing the solvent completely, the mixed crystal is heated together with a 3-6C alcohol, and separated into the solution part and the solid part by hot filtration, etc. Stevioside is obtained from the solution part, and the rebaudioside A can be prepared by washing and drying the solid part. |
