| 摘要 |
<p>An ion-exchange system and method is provided for isolating glycosylated hemoglobin (HB Al) from other hemoglobins in human blood together with a quantitative determination of glycosylated hemoglobin. Separation of the glycosylated hemoglobin is accomplished by the ion-exchange system with little temperature dependence in the range of from about 15o-37oC and without rigid control of pH and ionic strength. The ion-exchange system lowers the pH of the human blood to about pH = 6.9 through the use of an organic buffer preferably a zwitterionic buffer. The ion-exchange system also contains a cation-exchange resin. The preferred composition of the ion-exchange system contains about 0.05 molar 3-(N-morpholino) propanesulfonic acid as the buffer and carboxy-methyl dextran as the ion-exchange resin present in an amount of from about 30 milliequivalents to about 50 milliequivalents of binding capacity per liter thereof.</p> |
