| 摘要 |
An immuno-assay of a specimen of a serum or the like to determine immune complexes, includes producing on a plastic base a layer of a non-proteinaceous, non-ionic polymer which will adhere to the plastic base and has the capability of absorbing immune complexes of the specimen, placing a specimen on the layer and treating the layer to produce an indication of the amount of immune complexes. The polymer may be polyethylene glycol, dextran, polyvinyl chloride, a polymeric polyol or an adduct of polyethylene glycol. Washing with conventional solutions, addition of an antihuman IgG coupled with an enzymen and addition of a substrate reactive therewith, are similar to the ELISA test, with color measurement as by spectrophotometer. Or, the addition of anti IgG-I<125> and measurement by a scintillation counter may be used. The ethylene glycol may range in molecular weight from 2,000 to 20,000, although 6,000 to 8,000 is preferred. A product for use in such an assay is a plate P having wells (20) or a test tube T formed of plastic, polystyrene and polyvinyl chloride being preferred, with a layer (21, 31) of such non-proteinaceous, non-ionic layer on the plate wells (20) or the cavity of the test tube T. This product, if it is not to be used immediately, should be provided with a layer or film (22) protecting the polymer layer from the air. |
