Cell-driven viral transfer in Eukaryotes.

摘要

Novel method, cells and compositions are provided involving transforming B-lymphocytes to provide immortalization for continuous production of monoclonal antibodies to a predetermined ligand. T-cell free B-lymphocytes are combined with an-Epstein-Barr virus transformed cell sensitive to a cytotoxic agent which does not significantly affect the B-lymphocytes under conditions where the sensitive EBV transformed call acting as the transfer agent is killed and efficiently transforms the B-lymphocyte recipient cells with EBV. The EBV transformed B-lymphocyte cells are amplified and cloned, the desired clones isolated in accordance with conventional techniques and then used for production of monoclonal antibodies. <!-- EPO <DP n="2"> -->@ Implants (50) may be placed in animals in the field with a system including an implanter (10) and cartridge-like container (30) for a plurality of implants. The cartridge is transparent and encloses a plurality of elongated implants arranged side-to-side, transverse to the longitudinal axis of the cartridge. The implants may be moved manually (33) along the longitudinal axis of the cartridge toward one end and stopped in alignment parallel with the end (39) of the cartridge and with one implant aligned with a pair of openings (37,3S) in the cartridge, one opening at each end of the implant. The implanter carries a sharpened tubular needle (25) and a plunger, slidable along an axis aligned with the central axis of the tubular needle. The implanter has an opening (20) into which the cartridge may be fitted and retained. The implanter and cartridge have interfitting surfaces (28a, 28b, 27, 31c, 31d) to position the cartridge with its pair of openings lying upon the central axis of the hollow needle and to retain the cartridge in the implanter so that the plunger may be passed repeatedly through the pair of openings in the cartridge and move the implants, one after the other, through the sharpened tubular needle (25) into the animals. The implants thus are protected from contamination until implantation.