VACCINE AND METHOD OF MAKING

摘要

00014IA Culturing Streptococcus agalactiae in a nutrient broth containing at least 10 g/l of glucose while maintaining the pH at 6-8 by the addition of alkaline material produces polysaccharide antigen including a fraction having a molecular size from 8-11 x 105d. By employing a broth free from constituents having a molecular size above 105, the antigens can be separated from the broth in high yield after harvesting the organisms. Vaccination of humans with Streptococcus agalactiae polysaccharide antigens having molecular sizes above 5 x 105 produces in selected individuals specific antibody levels above 200 ug/ml of antisera; globulins fractionated from such antisera contain most of the antibody and can be used for passive immunization.